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Biocell Technology
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Image Search Results
Journal: bioRxiv
Article Title: Capturing limbal epithelial stem cell population dynamics, signature, and their niche
doi: 10.1101/2020.06.30.179754
Figure Lengend Snippet: (A) Whole-mount immunostaining of corneas was performed to label blood (Cd31) and lymph (Lyve-1) vessels of 2-3 months old Krt15-GFP mice. (B-C) Whole-mount immunostaining of corneas of immunodeficient mice (SCID) or controls to identify qLSCs (Cd63 + or Gpha2 + ) or T cell populations (Cde3 + and Cd4 + , or FoxP3-GFP + transgene) or dividing cells (Ki67 + ). (D) Quantification of Ki67 expression in different limbal zones. (E) Six-days post subconjunctival injection of anti-Cd25 antibody (inhibits regulatory T cells) to Krt15-GFP mice, whole mount immunostaining of the indicated qLSC markers was performed. (F) Quantification of EdU expression in different limbal zones. (G-H) Corneal epithelial debridement was performed in 2-month old mice and fluorescein dye stain was performed to follow wound closure. Typical images are shown in (G) and quantification in (H). Nuclei were detected by DAPI counter staining (C, E). Scale bars are 50 μm. Statistical significance was calculated using t-test (*, p-value<0.05). Abbreviations: Cj., Conjunctiva; Peri., Periphery.
Article Snippet: For CD4 + FoxP3 + regulatory T cell depletion, subconjunctival injection of 30 µl (250 µg)
Techniques: Immunostaining, Expressing, Injection, Staining